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The biotechnology portion of this program generated a great deal of data. Hundreds of marine bay mussels (Mytilus spp.) were collected in Moss Landing Harbor (Moss Landing, CA) over the span of 5 years. A few mussels were also collected from a site in Palo Alto, CA. Each was cataloged, measured, and assayed for three genetic loci. The invasive mussel (Mytilus galloprovincialis) could be differentiated from the morphologically indistinguishable native (Mytilus trossulus) using these genetic markers. Differences at two nuclear loci (ITS and Glu) could be distinguished on agarose gels. In addition, a mitochondrial locus (CO3) was sequenced in both direction for each sample.
A full listing of ALL mussel samples and corresponding shell size, images of gels, and DNA sequencing trace files, are found in this matrix. This remains a work in progress.
The first two digits in each sample name correspond to the year the mussel was collected and analyzed. The size is a measure of the shell at its greatest length. The DNA Gel shows a sample of extracted genomic DNA for assessing the quantity and quality. The Glu Gel has samples of PCR products after amplification with Glu primers. The ITS Gel has the resultant bands after PCR amplification with ITS primers and subsequent restriction enzyme digestion with Hha I. DNA sequence trace files are provided for both the forward (F) and reverse (R) direction of the CO3 PCR product.
To view trace files:
PC - Right Click to Download ab1 sequence trace then use Chromas to open the file.
Mac - Click on link and choose “Save File” then use FinchTV to open the file.
