Download Chromas by right-clicking on the link and saving the program to your desktop.
Download the example sequence file by right-clicking on the link and saving the file to your desktop.
Open Chromas.
Select FILE --> OPEN --> example.abi
Recall how sequencing machines operate and how the program has predicted nucleotides based on flourescent trace peaks.
Select EDIT --> COPY SEQUENCE --> FASTA FORMAT
Open Notepad.
Select EDIT --> PASTE
Be careful with the next two steps...
Use Ctrl-F to find the first "N," and delete the sequence up to that N.
Use Ctrl-F to find the next "N," and delete the sequence after that N.
Select FILE --> SAVE AS --> example_fasta.txt
Highlight the whole sequence and select EDIT --> COPY
Google "NCBI"
Click "Blast" and then "Nucleotide-Nucleotide Blast"
Paste your sequence into the search window and click "submit."
Wait the requisite number of seconds and then click "format."
What sequences matched the one from the trace?
