| Marine Biotechnology and
Bioinformatics Summer Workshop Schedule Overview |
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| WEEK 1 |
Begin Biotechnology and Wet Lab / Intro to
Bioinformatics / Intro to PBL Teaching |
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Monday |
Tuesday |
Wednesday |
Thursday |
Friday |
| 9:00 AM |
Overview & intros & evaluations |
Collect mussels (8 am = 0 tide) |
Finish DNA extractions |
Restriction digestion PPT & ITS digest set up |
Set up Glu PCRs |
| 10:00 AM |
Pretest |
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ITS digest paper exercise |
Glu primer paper exercise |
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Break |
Break |
Break |
Break |
Break |
| 11:00 AM |
Tracking mussels presentation, part I |
Mussel dissection review & dissect mussels |
Load DNA gel |
ITS digest on website and virtual gel |
Glu primer computer exercise |
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Lunch |
Lunch |
Lunch |
Lunch |
Lunch |
| 1:00 PM |
Tracking mussels presentation, part II |
DNA extraction PPT |
Quantifying DNA PPT & analyze DNA gel |
Load ITS gels |
Load Glu gels |
| 2:00 PM |
General lab skills & lab conduct discussion |
Gel electrophoresis PPT & gel loading exercise |
Quantify DNA and make working stock |
Webquest and project - based learning |
DNA seq. viewing & editing with Chromos |
| 3:00 PM |
Practice lab skills pipetting exercise |
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Webquest and PBL |
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Break |
Break |
Break |
Break |
Break |
| 4:00 PM |
Mussel dissection PPT |
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PCR PPT & Set up ITS PCR |
Webquest and PBL |
Webquest and PBL |
| 4:30 PM |
Review today and plan tomorrow |
Review today and plan tomorrow |
Review today and plan tomorrow |
Review today and plan tomorrow |
View Glu gels |
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View ITS gels |
Review today and plan Monday |
| 5:00 PM |
End |
End |
End |
End |
End |
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| KEY: |
Out in the Field |
Biotechnology |
Bioinformatics |
Wet Lab |
Teaching & Learning |
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| Week 2 |
Continue DNA Biotechnology / Begin Bioinformatics /
Write Lesson Plans |
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Monday |
Tuesday |
Wednesday |
Thursday |
Friday |
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| 9:00 AM |
Set up CO3 PCRs |
Load aliquot on gel |
Align sequences w/ Clustal |
Compare nucleotide & protein trees |
Start working with own sequences |
| 10:00 AM |
Mitochondria vs. nucleus PPT & exercise |
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View 3D proteins - Cn3D |
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Break |
Break |
Break |
Break |
Break |
| 11:00 AM |
Paper DNA priming exercise |
Prep for commercial DNA sequencing discussion &
DNA sequencing PPT |
Creating phylogenetic trees w/ Drawtree |
High-end bioinformatics |
continue |
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Lunch |
Lunch |
Lunch |
Lunch |
Lunch |
| 1:00 PM |
Load CO3 PCR gels |
View and analyze gel |
Translation & Open Reading Frames-ORF finder |
Intro to Japanese lesson study |
continue |
| 2:00 PM |
Are whales hippos? exercise |
Genbank and Blast searches |
Align Proteins w/ Clustalx |
Use of camcorders |
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| 3:00 PM |
Break |
Break |
Break |
Break |
Break |
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Lesson planning on related thematic topics |
Lesson planning on related thematic topics |
Lesson planning on related thematic topics |
REFLECTION TIME |
Scope and sequence for lessons |
| 4:00 PM |
Review today and plan tomorrow |
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Use of digital cameras |
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Using Skype for Web conferencing |
| 4:30 PM |
View CO3 gels |
Review today and plan tomorrow |
Review today and plan tomorrow |
Review today and plan tomorrow |
Review today and plan week 3 |
| 5:00 PM |
End |
End |
End |
End |
End |
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Special Evening Dinner Event |
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| KEY: |
Out in the Field |
Biotechnology |
Bioinformatics |
Wet Lab |
Teaching & Learning |
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Technology Topics |
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| Week 3 |
Teachers Implement Group Lesson Plans with Students |
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Monday |
Tuesday |
Wednesday |
Thursday |
Friday |
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| 9:00 AM |
Lesson Group 1: Collecting Mussels |
Lesson Group 2: Dissection in Wet Lab |
Lesson Group 3: Gel electrophoresis |
Field Trip to Biotechnology Company for everyone |
Lesson Group 4: Invasive Marine Species |
| 10:00 AM |
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| 11:00 AM |
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Lunch |
Lunch |
Lunch |
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Lunch |
| 1:00 PM |
Hands-on Learning Activity |
Hands-on Learning Activities |
Hands-on Learning Activities |
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Hands-on Learning Activities |
| 2:00 PM |
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| 3:00 PM |
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Break |
Break |
Break |
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Break |
| 4:00 PM |
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| 4:30 PM |
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| 5:00 PM |
End |
End |
End |
End |
End |
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| KEY: |
Out in the Field |
Student's Wet Lab |
Student's Activity |
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